Icosahedral Image Processing Projects

Current

• Icosahedral Tumbleweed provides easy, efficient, and routine determination of icosahedral virus structures from electron micrographs.

Post-Doctoral

• Review: Icosahedral Virus Structure Determination
• Multiresolution Based Approach to Automatic Particle Selection
• Determination of Amplitude Contrast and Envelope Decay Functions Using Solution X-ray Scattering

Pre-Doctoral

• Orientation Estimation
• PTOOL: Automatic Particle Selection Software
• Automatic Particle Selection

References

Peer Reviewed

1. C. Teschke, A. McGough, and P. Thuman-Commike (2003). Penton Release from P22 Heat-Expanded Capsids Suggests Importance of Stabilizing Penton-Hexon Interactions during Capsid Maturation. Biophysical J., 84:2585-92.
2. Z. Zhang, B. Greene, P. Thuman-Commike, J. Jakana, P. Prevelige Jr., J. King, W. Chiu (2000). Visualization of the Maturation Transition in Bacteriophage P22 by Electron Cryomicroscopy. Journal of Molecular Biology, 297: 615-626.
3. P. Thuman-Commike and W. Chiu. (2000). Reconstruction Principles of Icosahedral Virus Structure Determination Using Electron Cryomicroscopy. Micron, 31:687-71.
4. P. Thuman-Commike, H. Tsuruta, B. Greene, P. E. Prevelige Jr., J. King, and W. Chiu. (1999). Solution X-Ray Scattering Based Estimation of Electron Cryomicroscopy Imaging Parameters for Three-Dimensional Reconstruction. Biophysical Journal, 74, 2249-2261.
5. P. Thuman-Commike and W. Chiu. (1997). Improved Common Line-Based Icosahedral Particle Image Orientation Estimation Algorithm.Ultramicroscopy, 68, 231-255.
6. P. Thuman-Commike and W. Chiu. (1996). PTOOL: A Software Package for the Selection of Particles from Electron Cryomicroscopy Spot-scan Images. Journal of Structural Biology, 116:41 - 47.
7. P. Thuman-Commike and W. Chiu. (1995). Automatic Detection of Spherical Particles from Spot-scan Electron Microscopy Images. Journal of the Microscopy Society of America, 1, 191-201.

Conference Proceedings & Abstracts

• A. Saad, W. Chiu, and P. Thuman-Commike. (1998). Multiresolution Approach to Automatic Detection of Spherical Particles from Electron Microscopy Images. Proceedings of the IEEE 5th International Conference on Image Processing. Chicago, IL, W8-10.
• P. Thuman-Commike and W. Chiu. (1997). Three-Dimensional Reconstruction of Moisy Electron Microscopy Virus Particle Images. Proceedings of the IEEE 4th International Conference on Image Processing, volume I, 173 - 176.
• P. Thuman-Commike and W. Chiu. (1995). Detection of Virus Particles in Noisy Spot-Scan Electron Cryomicroscopy Images. Proceedings of the IEEE 2nd International Conference on Image Processing, volume III, 169 - 171.
• W. Chiu, J. Jakana, Z. Hong Zhou and P. Thuman-Commike. (1995). 400keV Electron Cryomicroscopy and Computer Reconstruction of Spherical Viruses. Microscopy Society of Canada, volume XXIII, 16 - 17.