Bacteriophage Projects

Current

• Structural Comparison of Bacteriophages Sf6 and P22

Recent

• Structural Studies of Heat Expanded Bacteriophage P22 Procapsids

Post-Doctoral

• Identification of Additional Coat/Scaffolding Interactions in a Bacteriophage P22 Mutant Defective in Maturation.
• Visualization of the Maturation Transition in Bacteriophage P22 with Zhixian Zhang
• Localization of the Coat/Scaffolding Interface: Mechanism of Scaffolding Directed Procapsid Assembly
• T = 4 and T = 7 Procapsids Assembled in the Absence of the Scaffolding Protein

Pre-Doctoral

• Three-dimensional Structure of Scaffolding-Containing Phage P22 Procapsids by Electron Cryo-microscopy at 19 Å resolution

References

1. C. Teschke, A. McGough, and P. Thuman-Commike (2003). Penton Release from P22 Heat-Expanded Capsids Suggests Importance of Stabilizing Penton-Hexon Interactions during Capsid Maturation. Biophysical J., 84:2585-92.
2. Z. Zhang, B. Greene, P. Thuman-Commike, J. Jakana, P. Prevelige Jr., J. King, W. Chiu (2000). Visualization of the Maturation Transition in Bacteriophage P22 by Electron Cryomicroscopy. Journal of Molecular Biology, 297: 615-626.
3. P. Thuman-Commike, B. Greene, J. Jakana, A. McGough, P. Prevelige Jr., W. Chiu (2000). Identification of Additional Coat-Scaffolding Interactions in a Bacteriophage P22 Mutant Defective in Maturation. Journal of Virology. 74:3871-3873.
4. P. Thuman-Commike, B. Greene, J. A. Malinski, M. Burbea, A. McGough, W. Chiu, P. E. Prevelige Jr. (1999). Mechanism of Scaffolding-Directed Virus Assembly Suggested by Comparison of Scaffolding-Containing and Scaffolding-Lacking P22 Procapsids. Biophysical Journal, 76: 3267-3277.
5. P. Thuman-Commike, B. Greene, J. Malinski, J. King, and W. Chiu. (1998). Role of the Scaffolding Protein in P22 Procapsid Size Determination Suggested by T = 4 and T = 7 Procapsid Structures. Biophysical Journal, 74, 559 - 568.
6. P. Thuman-Commike, B. Greene, J. Jakana, B. V. V. Prasad, J. King, P. Prevelige Jr., and W. Chiu. (1996). Three-Dimensional Structure of Scaffolding-containing Phage P22 Procapsids by Electron Cryo-microscopy. Journal of Molecular Biology, 260:85-98.
7. C. Teschke, A. McGough, and P. Thuman-Commike. (2003). P22 Heat-Expanded Capsids Suggests Importance of Stabilizing Penton-Hexon Interactions during Capsid Maturation. Biophysical Journal, 84:2585-2592.